![]() InĪddition, galectin-1 RNA interactome data was also integrated to explore the Performed to comprehensively investigate gene expression and AS levels. To address this question, LGALS1 was silenced in human umbilical veinĮndothelial cells (HUVECs) and whole transcriptome sequencing (RNA-seq) was then Galectin-1 modulates the transcriptome profiles and interacts with the RNA Transcriptomes profile of HeLa cells, including the AS patterns of transcripts,īy interacting with spliceosomes. As an RBP, galectin-1 was found to regulate the Influencing the expression of genes including VEGFA, EGR1, and Participate in angiogenesis by binding to specific transcripts and thereby High throughput sequencing (iRIP-seq) techniques. In a previous study, we investigated the potentialįunctions of galectin-1 target RNAs using improved RNA immunoprecipitation and This indicates that galectin-1 can modulate the fate and function of RNAsīy direct interaction. Protein (RBP) by extracting polyadenylated RNAs interactomes from HeLa cells Interacting with proteins, galectin-1 could also serve as a potential RNA binding Inhibit angiogenesis in many diseases, including cancer. Thus, targeting galectin-1 may be a therapeutic strategy to Inducing the phosphorylation of VEGF receptor 1 (VEGFR1) and VEGF receptor 2 A previous study demonstrated that galectin-1 and galectin-3 togetherĮnhanced the growth and tube formation of endothelial cells (EA.hy926) by Programs by counteracting the synthesis of proinflammatory cytokines and immuneĬells. Galectin-1 has been shown to modulate inflammatory Differential expression of galectin-1 is associated withĪ wide range of biological activities, suggesting that it plays a critical role ![]() Galectin-1 is a carbohydrate-binding protein with an affinity for Was first reported in 2006 as playing a critical role in tumor angiogenesis. Galectin-1, encoded by lectin galactoside-binding soluble-1 ( LGALS1), These results indicate that theĮxpression levels of angiogenic factors may be regulated by other proteins whoseįunctions in the pathogenesis of angiogenic disorders require further A recent study found that the environment surroundingĮxtracellular matrix (ECM) compression could regulate microvascular growth and With angiogenic disorder, and is also involved in the regulation of tumorĪngiogenesis. Transforming growthįactor-beta (TGF-beta) is highly expressed in the smooth-muscle cells of patients ![]() Spectrum of biological functions, including angiogenesis. įibroblast growth factors (FGFs) and their receptors (FGFRs) regulate a broad Their receptors play key roles in normal and pathological angiogenesis. Vascular endothelial growth factors (VEGFs) and Due to their critical roles in variousĪngiogenesis-related disorders, several key proteins have been reported to Īnti-angiogenesis could serve as an effective strategy for treating cancers Īnd ischemic diseases. Of many diseases, including cancers and various other disorders. Indicate that galectin-1 could serve as a therapeutic target for futureĪngiogenesis, also known as neovascularization, is critical for the progression Galectin-1 and the molecular mechanisms that underlie angiogenesis. These findings expand our understanding of the functions of Transcriptional and post-transcriptional levels, probably by binding to the Conclusions: Our resultsĭemonstrate that galectin-1 can regulate angiogenesis-related genes at Those enriched in the angiogenesis pathway. Galectin-1, hundreds of RASGs were found to be bound by galectin-1, including Furthermore, based on our previously published RNA interactome data for Regulated AS genes (RASGs) were found to be enriched in focal adhesion and in theĪngiogenesis-associated vascular endothelial growth factor (VEGF) signaling (ES) and intron retention, and inhibition of cassette exon events. Used to analyze dysregulated AS profiles, such as the promotion of exon skipping These were validated by reverse transcription and quantitative polymerase chain reaction (RT-qPCR) experiments. ![]() Were primarily enriched in angiogenesis and inflammatory response pathways, and (siLGALS1), comprising 604 up- and 847 down-regulated DEGs. Results: A total of 1451 differentiallyĮxpressed genes (DEGs) were found to be regulated by silencing LGALS1 Integrated to explore how galectin-1 might regulate gene expression andĪlternative splicing (AS). LGALS1 was silenced in human umbilical vein endothelial cells (HUVECs)Īnd whole transcriptome sequencing (RNA-seq) was then performed to investigate Underlying mechanisms need further clarification. ( LGALS1), has critical roles in the regulation of angiogenesis, but the Galectin-1, encoded by the lectin galactoside-binding soluble-1 gene Therefore its dysregulation can cause various diseases, including cerebrovascularĭisease. Background: Angiogenesis is essential for tissue development, and
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